Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R128G | reduces the AGT repair efficiency, no smaller effects with the O6-benzylguanine substrate than the 6-O-methylguanine | Homo sapiens |
R128L | reduces the AGT repair efficiency, smaller effects with the O6-benzylguanine substrate than the 6-O-methylguanine | Homo sapiens |
Y114F | reduces the AGT repair efficiency, smaller effects with the O6-benzylguanine substrate than the 6-O-methylguanine | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 6-O-benzylguanine + [protein]-L-cysteine | AGT binds and scans DNA rapidly, flips O6-alkylG residues, transfers the alkyl group in a chemical step that is not rate-limiting in the case of 6-O-benzylguanine and releases the dealkylated DNA | Homo sapiens | DNA lacking 6-O-benzylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
DNA containing 6-O-methylguanine + [protein]-L-cysteine | AGT binds and scans DNA rapidly, flips O6-alkylG residues, transfers the alkyl group in a chemical step that is rate-limiting in the case of 6-O-methylguanine and releases the dealkylated DNA | Homo sapiens | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AGT | - |
Homo sapiens |
O6-alkylguanine-DNA alkyltransferase | - |
Homo sapiens |